A high number of immune cells may be predictive of disease prognosis in cancers and response to therapies. Therefore, understanding the immune cell phenotypes present within cancerous tissue can be valuable in developing strategies that aid in the treatment of cancer. In this webinar we will discuss the development and application of multispectral imaging for the characterization of the tumor microenvironment and analysis of individual immune cell subsets pre and post therapy in breast cancer samples.
- An overview of why multiplexing is emerging as a critical technique in characterizing clinically relevant immuno-oncology biomarkers.
- Learn the methodology of optimizing multiplex panels.
- Explore the multiplicity of phenotypes shown from multiplex panels.
- Review case study results showing the recruitment of T helper cells, histiocytes and T-cells post-cancer treatment detected by increase in FoxP3 and CD4.
- Learn the different applications that multiplex phenotyping can aid in analysis of high dimensional human tissue.
Speaker: Bhavika Patel, Ph.D.
Bhavika received her BS in Psychology with a minor in Molecular and Cellular Biology and Chemistry from the University of Illinois. She later went on to complete her Ph.D. in Neuroscience from Iowa State University and is currently a Scientist at Lanterne Dx. Bhavika has over 8 years of experience with immunohistochemistry and fluorescent microscopy and now supports Lanterne Dx’s multiplex imaging and digital image analysis using various platforms.
Speaker: Brenna Dennison
Brenna received her BS in Biology with a minor in Psychology and Criminal Justice from the University of Colorado Denver. She later went on to complete her PhD in Cell Biology, Stem Cells, and Development from University of Colorado, Anschutz Medical Campus and is currently a Scientist at Lanterne Dx.
Brenna has over 7 years of RNA in situ hybridization, immunohistochemistry, and immunofluorescence experience.